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. 2008 Jul 11;8:115. doi: 10.1186/1471-2180-8-115

Table 2.

Strains and plasmids used in this study

Strain or plasmid	Genotype or description	Source or reference
Bacterial Strains

E. coli

S17-1	hsdR17 (r_k^-m_k^-)recA RP4-2(Tc^r::Mu-Km^r::Tn7 Str^r)
TOP10	F^-mcrA Δ(mrr-hsdRMA-mcrBC) φ80lacΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL (Str^r) endA1 nupG	Invitrogen

F. columnare

C#2	Wild Type	[13]
AL-203-94	Wild Type	[13]
Fc14-56	Wild Type	[26]
94-060	Wild Type	[26]
1191-B	Wild Type	[26]
94-078	Wild Type	[26]
94-081	Wild Type	[26]
90-059	Wild Type	[26]
L90-659	Wild Type	[26]
92-002	Wild Type	[26]
C91-20	Wild Type	[26]
FcAS44	gldJ knockout mutant of C#2	This Study

Plasmids

pAMSTA39	PCR cloning vector with promoter-less gfp; Ap^rKm^r	This Study
pAS29	Promoter-less gfp containing E. coli-Flavobacterium shuttle vector; Ap^r(Em^r, Cf^r)	This Study
pAS36	P_map-gfp containing E. coli-Flavobacterium shuttle vector; Ap^r(Em^r, Cf^r)	This Study
pAS42	1400-bp fragment of gldJ in pCP29; Ap^r(Em^r, Cf^r)	This Study
pAS43	P_ompA-gfp containing E. coli-Flavobacterium shuttle vector; Ap^r(Em^r, Cf^r)	This Study
pCE320	gfp-containing E. coli-Borrelia burgdorferi shuttle vector; Ap^r	[46]
pCR4-TOPO	PCR cloning vector; Ap^rKm^r	Invitrogen
pCP11	E. coli – Flavobacterium shuttle plasmid; Ap^r(Em^r)	[21]
pCP29	E. coli – Flavobacterium shuttle plasmid; Ap^r(Cf^rEm^r)	[37]
pEP4351	λpir dependent R6K oriV; RP4 oriT; Cm^rTc^r(Em^r); Tn4351 mutagenesis vector	[53]
pCR4-TOPO	PCR cloning vector; Ap^rKm^r	Invitrogen