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. 2008 Jul 30;3(7):e2829. doi: 10.1371/journal.pone.0002829

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Ahn et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Microglial markers (CD11b or Iba-1) were co-expressed with albumin in all HMO6 cells (a) and human primary microglial cells (b). This observation was further confirmed by immunohistochemical staining of human fetal (c) and adult (d) brain tissues, in which albumin was co-expressed with CD11b or Iba-1. Anti-human-albumin antibody used for ICC and IHC does not have cross-reactivity with bovine albumin. Minimal co-localization of albumin and non-microglial markers was observed in other cell types of brain: MAP2 for neurons (e); GFAP for astrocytes (f); MBP for oligodendrocytes (g). Scale bars = 50 µm.