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KMC activity for a sample MV with continuously bound PSGL-1. Bound PSGL-1 (solid asterisks) and LFA-1 (diamonds) elements are fixed at the MV tip, whereas MAPK (circles) are fixed at the MV base. ADIN (triangles) and ADOUT (squares) diffuse between the base and tip, carrying the activation signal (solid symbols indicate activated state). Lines represent KMC bonds. LFA-1 activation is indicated by solid diamonds.
