TABLE 2.

Additional parameter and initial condition values for 1D simulations

Parameter	Description	Value	Comments
D_DAG	Diffusion coefficient for DAG	10 μm2/s	Similar to D_PIP2
D_IP3	Diffusion coefficient for IP3	283 μm2/s	(64)
D_PI	Diffusion coefficient for PI	10 μm2/s	Adapted from Haugh et al. (33)
D_PIP	Diffusion coefficient for PIP	10 μm2/s	Adapted from Haugh et al. (33)
D_PIP2	Diffusion coefficient for PIP2	10 μm2/s	Adapted from Haugh et al. (33) and Wagner and Tamm (34)
DAGd_PM_init	Initial DAG concentration	2000 mol/μm2	Model from Xu et al. (26)
DAGs_PM_init	Initial DAG concentration	2000 mol/μm2	Model from Xu et al. (26)
IP3d_Cyt_init	Initial IP3 concentration	0.16 μm	(30)
IP3s_Cyt_init	Initial IP3 concentration	0.16 μm	(30)
PId_PM_init	Initial PI concentration	142,857 mol/μm2	(26)
PIP2d_PM_init	Initial PIP2 concentration	4000 mol/μm2	(26)
PIP2s_PM_init	Initial PIP2 concentration	4000 mol/μm2	(26)
PIPd_PM_init	Initial PIP concentration	2857 mol/μm2	(26)
PIPs_PM_init	Initial PIP concentration	2857 mol/μm2	(26)
PIs_PM_init	Initial PI concentration	142,857 mol/μm2	(26)
To fit constructed 3D geometry
L_neck	Spine neck length	0.33 μm	Adapted from Harris and Stevens (29)
n	Spine density	14 μm−1	(29)
r_d	Dendrite radius	0.63 μm
r_neck	Spine neck radius	0.0418 μm	Adapted from Harris and Stevens (29)
r_spine	Spine head radius	0.34 μm	Adapted from Harris and Stevens (29)
stim_PM	Linear description of single spine in 1D	(x < 0.0367)*	Calculated
To fit experimentally derived 3D geometry
l_neck	Spine neck length	0.37 μm	Calculated
n	Spine density	11 μm−1	Calculated
r_d	Dendrite radius	0.6 μm	Calculated
r_neck	Spine neck radius	0.02 μm	Calculated
r_spine	Spine head radius	0.34 μm	Calculated
stim_PM	Linear description of single spine in 1D	(x < 0.0454)*	Calculated

*See ID geometries in “Purkinje ID” located under the user name “Brown” in the Virtual Cell database.