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SDS-PAGE and Western-blot of hNL3-cyt after nondenaturing size exclusion chromatography. hNL3-cyt elutes in several oligomeric states, which are stable under the conditions of SDS-PAGE. Lanes 1 and 2, SDS-PAGE of hNL3-cyt loaded at 2.4 mg/ml, and 4.8 mg/ml; lane 3, Western-blot of hNL3-cyt using anti-His antibody, which displays at least three distinct oligomeric states.
