FIGURE 2.

Kupffer's vesicle flow extracted from fluorescence data. (A) Particle tracks (N = 266) indicating the fluid streamlines of the steady-state flow within the KV. The three different track colors correspond to the superposition of tracks obtained at different time windows (3 × 15 s over a 45-s period) showing that tracks align over time, indicating a steady state. The particles exhibit a circular motion around the dorso-ventral direction. (B) Schematic display of the spatial orientation (antero-posterior, dorso-ventral, and left-right axis are represented in green, blue, and red, respectively) of the vesicle (red sphere). The black arrows indicate the counterclockwise rotation of the flow (when viewed from the dorsal side). d, dorsal; v, ventral; a, anterior; p, posterior, l, left; and r, right.