Figure 5.

Binding of ³H 9-cis-retinoic acid or ³H All-trans-retinoic acid to (His)₆jRXR. (A) 10 ng of fusion protein was incubated with ³H 9-cis-retinoic acid or ³H-all-trans-retinoic acid (purchased from DuPont/NEN) and competed or not with a hundred fold excess of unlabeled 9-cis-retinoic acid or all-trans-retinoic acid (Sigma) as indicated. A representative of five independent experiments done in triplicates is shown. SED is indicated. (B) A kinetic analysis of binding; insert shows the Scatchard analysis. K_d = 0.4 nM. ▴ are total binding, ■ are nonspecific binding and ♦ are specific binding.