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. Author manuscript; available in PMC: 2009 May 1.

Published in final edited form as: Cell Signal. 2008 Jan 11;20(5):892–906. doi: 10.1016/j.cellsig.2008.01.001

(A) After caspase-8 inhibitor (Z-IETD-FMK 20 µM) was pretreated for 30 min, followed by TRAIL treatment (200 ng/ml) for 4 h, various MAPKs were examined. Left panel: Western blot analysis. Right panel: The ratio of the phosphorylated MAPKs to total protein of corresponding MAPKs in TRAIL-treated DU-145 cells was set equal to 1, and the ratio of the phosphorylated MAPKs to total protein of corresponding MAPKs in TRAIL-treated DU-145 cells with caspase-8 inhibitor pretreatment was compared to this. Data are expressed as mean ± SE of the densitometry data from three independent experiments. (B) After caspase-8 was downregulated by siRNA of caspase-8, TRAIL was treated for the examination of various MAPKs. Left panel: Western blot analysis, C, control; T, TRAIL. Right panel: The ratio of the phosphorylated MAPKs to corresponding actin in TRAIL-treated si scrambled RNA-transfected cells was set equal to 1, and the ratio of the phosphorylated MAPKs to corresponding actin in TRAIL-treated si caspase-8 RNA-transfected cells was compared to this. Data are expressed as mean ± SE of the densitometry data from three independent experiments.

(A) After caspase-8 inhibitor (Z-IETD-FMK 20 µM) was pretreated for 30 min, followed by TRAIL treatment (200 ng/ml) for 4 h, various MAPKs were examined. Left panel: Western blot analysis. Right panel: The ratio of the phosphorylated MAPKs to total protein of corresponding MAPKs in TRAIL-treated DU-145 cells was set equal to 1, and the ratio of the phosphorylated MAPKs to total protein of corresponding MAPKs in TRAIL-treated DU-145 cells with caspase-8 inhibitor pretreatment was compared to this. Data are expressed as mean ± SE of the densitometry data from three independent experiments. (B) After caspase-8 was downregulated by siRNA of caspase-8, TRAIL was treated for the examination of various MAPKs. Left panel: Western blot analysis, C, control; T, TRAIL. Right panel: The ratio of the phosphorylated MAPKs to corresponding actin in TRAIL-treated si scrambled RNA-transfected cells was set equal to 1, and the ratio of the phosphorylated MAPKs to corresponding actin in TRAIL-treated si caspase-8 RNA-transfected cells was compared to this. Data are expressed as mean ± SE of the densitometry data from three independent experiments.