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. Author manuscript; available in PMC: 2009 Jun 9.
Published in final edited form as: Exp Cell Res. 2008 Mar 28;314(10):2076–2089. doi: 10.1016/j.yexcr.2008.03.012

Figure 3. Synuclein translocation to mitochondria is directly influenced by pHi.

Figure 3

Immunofluorescence images of SK-N-SH cells expressing α-synuclein clamped to various intracellular pHs with high K+ buffers/nigericin. Cells were incubated for 15 minutes before fixation with 3.7% formaldehyde in 0.1 M PO4, pH 7.2. Cells were labeled with antibodies to synuclein and BclXL. Note the partial redistribution of synuclein at pHi 6.0 and below; complete redistribution occurs at pHi 5.0. The green arrow shows one of several synuclein clusters that form at low pH. Bar: 10 µm.