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. 2008 Aug 8;4(8):e1000148. doi: 10.1371/journal.pgen.1000148

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A) Percentage of introns with the most common BP-like hexamer across eukaryotes. Dark colored bars show the percentage of introns with the most common branch-point hexamer; light colored bars show the additional introns allowing for two-fold degeneracy at one of the six sites. The left red bar separates species with ‘strong’ BP motifs (>50% of introns) from species with weaker BP motifs. The right red bar separate extremely intron-poor species (< 0.15 introns per gene). Asterisks (*) mark the two exception to the correspondence between the two variables (strong BP/intron-poor vs. weak BP/intron-rich). (B) Logarithm of total intron number (Y-axis) and percentage of introns showing the most common potential branch point motif with one site two-fold degeneracy (X-axis) are plotted for all fully sequenced studied genomes. The species cluster in two different groups (with two exceptions) - intron-rich/weak BP consensus, intron-poor/strong BP consensus. The two intron-poor species not showing strong consensus branch points, G. thetha NM and C. parvum, are labeled. The line corresponds to a lineal regression analysis, showing the negative correlation (r = −0.75) between intron numbers and branch point consensus conservation.