Table 1. Gene-specific primers used in this study.

Primer Name	Sequence (5′ to 3′)	Target Region
(1) MHCI-R3	ASGTAYYTCBBCAGCCACT	Initial exon 3 genomic amplification
(2) IgNC-F1	TGYGAGCTGAGGAAAGATGGGAGCATAG	3′ RACE
(3) IgNC-F2	TTACCARTGTGCTTATGATGGGAGGGAC	3′ RACE (Nested)
(4) MHC17H3-5R-R1	TTCATGCAGTCCAAAGGCAGCAG	5′ RACE; Genomic fragment
(5) MHC17H3-5R-R2	TCTTCTGCCTTGCTTCTGTCAAATATGGAG	5′ RACE (Nested)
(6) MHC1-Gr2-F1	ACCGAGAGGGTTGAGCTGGAGAG	Genomic fragment
(7) MHCI-Int-R1	TGAGGCTGASAGAGTGTAATCCTCCC	Iguaninae exon3/intron3 fragment
(8) MHC1-Int2-F2	AGRTGTRTGTAATATATCATCCAGG	Intron3/exon4 fragment (All Iguaninae)
(9) MHC1-R4	CAGRCYGGYRTGMTCCACRCGSCAC	Intron3/exon4 fragment (Amcr; Cosu)
(10) MHC1-R6	TCTCCTTGGGGTAGAAGCCRTC	Intron3/exon4 fragment (Cyco; Ctde; Cyca; Ctcl; Cyri)

Numbers before primer refer to location in the gene as indicated by arrows in Figure 1.

Footnote: See Materials and methods for Iguaninae taxa abbreviations.