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. 2008 Aug 6;3(8):e2875. doi: 10.1371/journal.pone.0002875

Figure 2. Rpi-blb1, Rpi-sto1, and Rpi-pta1 functionally interact with the avirulence protein IpiO.

Figure 2

The R-AVR interaction was reconstructed in a transient expression system in N. benthamiana [20], [21] by coinfiltrating equal mixtures of A. tumefaciens strains carrying pCB302-empty, -IpiO2, and -Avr3a with A. tumefaciens strains carrying (A) pBINPLUS-blb1, (B) –sto1, (C) –pta1 and (D) -R3a. A specific HR illustrates the identified R-AVR interactions between Rpi-blb1, Rpi-sto1, Rpi-pta1 and IPO2 and the positive control R3a-Avr3a. Pictures were taken at 8 dpi.