Figure 4. Characterization of rudra mutant flies.

(A) Real-time RT-PCR analysis of rudra transcript from w¹¹¹⁸, rudra^EY00723, and rdr^rescue flies that were infected with E.coli for various times. (B) Quantified Northern blotting data of Diptericin and rp49 expression in w¹¹¹⁸, rudra^EY00723 and rudra^rescue flies following infection with E.coli. (C) Survival assays were performed following infection of w¹¹¹⁸, rudra^EY00723 and LE¹¹²;LC^ΔE flies with E. carotovora carotovora. Infected animals were incubated at 29°C and the number of surviving flies were counted every 24 hours. Survival data is presented in Kaplan-Meier plots and significance was analyzed by log-rank test. (A) and (B) are representative of at least 3 independent experiments, while (C) is representative of 2 independent trials, with 60 or 100 animals.