Table 1. Rudra interacts with cytoplasmic domain of PGRP-LC by yeast two-hybrid.

Baits	LC WT	LC Δ172-212	LC Δ213-242	LC F218A	DmIKK	Empty vector
Prey: Rudra aa 30–197	++++	++	++	++	−	−

The cytoplasmic domain of PGRP-LC was used as bait and Rudra was used as the prey in yeast two-hybrid assays. Rudra interacted well with the full cytoplasmic domain of PGRP-LC and the yeast cells grew robustly on Ade selection plates. However, Rudra interacted weakly with several deletion and point mutants that alter the RHIM-like domain of PGRP-LCx. ++++, robust growth; ++, slow growth, − no growth.