. 2008 Jul 8;2:59. doi: 10.1186/1752-0509-2-59

Table 4.

Parameters estimation results of acetate and propionate enriched cultures for the different feeding conditions

Substrate	Acetate		Propionate		Mixture
Culture enrichment	Acetate	Propionate	Acetate	Propionate	Acetate	Propionate

Number of experiments used for parameters estimation/model validation	(7/3)	(1/0)	(1/0)	(3/1)	(1/0)	(1/0)

P/O ratio, d (mol-ATP/mol-NADH₂)	2.94 ± 0.16	1.08 ± 0.12	1.80 ± 0.19	0.94 ± 0.04	2.90 ± 0.27	1.78 ± 0.19

Maximum rates determined by metabolic model [C-mol/(C-mol.h)]

R_{6, max}	-	-	-	-	0.092 ± 0.017	0.094 ± 0.046
*R_{9, max}*	0.52 ± 0.03	0.13 ± 0.01	0.033 ± 0.005	0.053 ± 0.003	0.30 ± 0.03	0.18 ± 0.02
*R_{10, max}*	-	-	0.041 ± 0.004	0.052 ± 0.003	0.22 ± 0.02	0.13 ± 0.02

Maximum rates determined by MFA [C-mol/(C-mol.h)]

*R_{1, max}*	0.73 (0.01)	0.21 (0.02)	-	-	0.33 (0.07)	0.22 (0.05)
*R_{2, max}*	-	-	0.12 (0.02)	0.17 (0.02)	0.34 (<0.01)	0.23 (<0.01)
*R_{3, max}*	-	-	0.049 (0.005)	0.074 (0.005)	0.074 (0.018)	0.059 (0.016)
*R_{6, max}*	0.18 (<0.01)	0.07 (<0.01)	0.016 (<0.001)	0.021 (0.003)	-	-
*R_{8, max}*	0.24 (0.02)	0.11 (<0.01)	0.094 (0.006)	0.13 (0.02)	0.19 (<0.01)	0.22 (0.01)
*R_{PHB, max}*	0.52 (0.03)	0.13 (0.01)	0.011 (0.003)	0.018 (0.001)	0.14 (0.02)	0.090 (0.005)
*R_{PHV, max}*	-	-	0.035 (0.006)	0.086 (0.006)	0.26 (0.03)	0.22 (0.03)
*R_{PH2MV, max}*	-	-	0.026 (0.003)	-	0.12 (0.01)	-

The confidence bounds in parenthesis were obtained by error propagation of the metabolic model parameters

f_{PHA, max}= 2.47 C-mmol/C-mmol [10]; a = 3.85 (dimensionless) [10]; m_ATP= 0.02 mol-ATP/(C-mol.h) [39]; K_S= 0.062 C-mmol/l [39]; K_N= 0.56 N-mmol/l [40]