Figure 2.

Gel filtration chromatography of the [¹⁴C]Gal-labeled oligosaccharides from exoR galE derivatives. All strains carried the exoR95∷Tn5 mutation so that the exo genes are expressed constitutively and a galE mutation, which blocks UDP-Gal synthesis, so that the strains cannot synthesize succinoglycan or succinoglycan intermediates until UDP-[¹⁴C]Gal is introduced into the cell by electroporation. (A) The lipid linked oligosaccharides extracted from electroporated exoR galE cells with solvent 1203 (23), subjected to mild acid hydrolysis, and chromatographed using a Bio-Gel P4 column. (B) The Bio-Gel P4 chromatography of oligosaccharides isolated from the culture supernatants of electroporated exoR galE cells. (C) The same material as in B but chromatographed through a Bio-Gel P6 column. (D–F) The same analyses as A, B, and C, respectively except that exoR galE exoP cells were used. (G–I) The same analyses as A, B, and C, respectively, except that exoR galE exoT cells were used. (J–L) The same analyses as A, B, and C, respectively but exoR galE exoQ cells were used.