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. 2003 Oct 24;5:197–203. doi: 10.1251/bpo62

Fig. 4. A combination of four proinflammatory mediators is required for full maturation of FastDC.

Fig. 4

Monocytes were cultured with GM-CSF plus IL-4 for 48 hours and stimulated with either the combination of all four proinflammatory mediators or with each of the mediators alone or with soluble CD40L trimer for the last 24 hours. Expression of mature DC surface markers was determined by FACS (top) and culture supernatants were harvested for measurement of total IL-12 (IL-12 (p40) and IL-12 (p70), bottom). Data represent means ± SEM of four experiments with different donors.