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. 1998 Nov 10;95(23):13501–13506. doi: 10.1073/pnas.95.23.13501

Figure 1.

Figure 1

SV40 enhancer-activated transcription is not stimulated in the presence of the deacetylation inhibitor TSA. Nuclease S1 analysis of RNA from mouse B78 cells transfected with the reporter constructs SP1*-TATA-MG and SP1-TATA-MG-SVE in the presence and absence of the deacetylase inhibitor TSA. Cells were transfected with 12.5 μg of SP1*-TATA-MG and different amounts of SP1-TATA-MG-SVE (lanes 1 and 2, 12.5 μg; lanes 3 and 4, 2.5 μg; lanes 5 and 6, 0.5 μg). Half of the transfected cells was treated with TSA (100 ng/μl) for 16 hr, and RNA from treated and nontreated cells was subjected to nuclease S1 analysis using an end-labeled probe derived from the SP1*-TATA-MG template (see Materials and Methods). The transcribed regions of SP1-TATA-MG-SVE (SVE) and SP1*-TATA-MG (MG*) differ through a 30-nt sequence; therefore, transcripts can be distinguished by using the same probe.