Figure 3.

Interaction of Tat with p300 in vitro. The indicated GST fusion proteins (5 μg) on agarose beads were incubated with ³⁵S-labeled p300 or hTAF32 (negative control). Bound proteins are expressed as percentages of the input radiolabeled protein. (a) GST-Tat binds specifically to p300. (b) Effects of truncations at the C terminus of Tat. (c) Binding of p300 to Tat mutants. Immobilized GST fusion proteins (1.5, 3, and 6 μg) were incubated with ³⁵S-labeled p300. Samples were processed as in a.