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Photoaffinity crosslinking of Gal4 to TBP and to Gal80. 125I-APDP-Gal4 was incubated with purified Gal80 (lane 2), with purified yTBP (lane 3), and with both proteins (lane 4). In each case, after UV-induced crosslinking, the label was transferred by treatment with a reducing agent as described in ref. 9 (the minor band in lanes 2 and 4 is a degradation product of Gal80).
