Figure 3.

MRG1 is a nuclear protein with transcriptional activation function. (A) Cellular localization of MRG1 protein. pEGFP vector (CONTROL) or pMRG1-EGFP hybrid plasmid (MRG1) was transfected into NIH 3T3 by electroporation. Living cells were observed by fluorescence microscopy at 48 hr posttransfection. (B) Transcriptional activation of MRG1. NIH 3T3 cells were cotransfected with pM (negative control), pM3VP16 (positive control), GAL4BD-VP16 fusion protein), or pMMRG1 (GAL4BD-MRG1 fusion protein) and reporter plasmid pG5CAT. The assay for CAT activity was performed 48 hr posttransfection. All transfections were normalized according to β-galactosidase activity, which was used as an internal control for transfection. Data (X ± SEM) shown are representative of three independent experiments.