Table 1.
Plasmid | No. of cells | Agar cloning in
|
|
---|---|---|---|
−mIL-9 | +mIL9 | ||
pMRG1 | 105 | 64.7 ± 5.7 | 1.0 ± 0.0 |
106 | 625.7 ± 18.9 | 9.7 ± 0.6 | |
107 | 5,874.7 ± 61.6 | 99.0 ± 2.7 | |
pmIL-9 | 105 | 59.3 ± 7.8 | 0.67 ± 0.6 |
106 | 596.3 ± 10.5 | 11.7 ± 0.6 | |
107 | 5,927.0 ± 77.2 | 291.3 ± 10.0 | |
pCDNA3 | 106 | 508.0 ± 5.6 | 0 |
107 | 5,138.7 ± 105.0 | <0.66 ± 1.6 | |
None | 106 | 549.7 ± 22.9 | 0 |
107 | 5,606.3 ± 20.6 | <0.33 ± 0.6 |
Mean ± SEM of clones from three plates grown with G418 (pMRG1-, pmIL-9-, or pCDNA3-transfected cells) or without G418 (untransfected cells).