Figure 4.

Competition-binding assay of ³²P-labeled ɛC-oligomer (Fig. 1, seq. 1) with the same unlabeled ɛC-oligomer and with unmodified oligomer (Fig. 1, seq. 6); 0.02 pmol ³²P-labeled ɛC 25-mer was incubated at room temperature for 30 min with 2.5 μl of Mono-S fractions in the presence of increasing amounts of unlabeled ɛC- (lanes 3–5) or unmodified (lanes 6–8) oligomers. Lane 1 is the control without protein, and lane 2 is with protein but no competitor. For symbols, see Fig. 3. Note that the unmodified 25-mer lacking ɛC did not compete for ɛC binding (lanes 6–8).