Figure 7.

Time course of cleavage of G/T (Fig. 1, seq. 4) and ɛC⋅G 25-mer (Fig. 1, seq. 1) by using DNA mismatch N-glycosylase of the thermophilic archaeon M. thermoautotrophicum THF; 0.05 unit of enzyme was incubated with 20 fmol oligomer substrates at 65°C for indicated times under the conditions described in Materials and Methods. In each case, the furthest migrating bands are the expected cleavage products. The 7-mer marker is shown on the left. The size of the substrate oligomers used are indicated also by their migration.