Figure 1.

SecA36 can drive in vitro translocation of proOmpA into ΔsecG IMV. Urea-washed IMV, either from secG⁺ strain (GN67; A and C) or the ΔsecG∷kan mutant (GN68; B and D), were combined with wild-type SecA protein (A and B) or the SecA36 mutant protein (C and D) for translocation of ³⁵S-labeled proOmpA. The translocation reaction was allowed at 37°C (lanes 1–5) or at 20°C (lanes 6–10). Samples were withdrawn at 0.5 (lanes 1 and 6), 1 (lanes 2 and 7), 2 (lanes 3 and 8), 5 (lanes 4 and 9), and 10 (lanes 5 and 10) min after initiation of reaction, treated with trypsin, and subjected to SDS/PAGE. Translocated OmpA protein was visualized by phophorimager.