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. Author manuscript; available in PMC: 2008 Jul 25.
Published in final edited form as: Cell. 2006 Jan 13;124(1):147–159. doi: 10.1016/j.cell.2005.12.018

Figure 5. Sizzled, but not SzlD92N Mutant, Inhibits the Proteolytic Activity of Xlr on Its Natural Substrate Chordin.

Figure 5

(A) Diagram of Chordin protein structure and antibody recognition sites.

(B) Specific proteolysis of Chd (20 nM) by Xlr enzyme can by inhibited by increasing concentrations of Szl-Fc (lanes 3–8, 4–100 nM).

(C) Increasing concentrations of SzlD92N/Ogon-Fc do not inhibit Xlr activity.

(D) Dorsalizing biological activity of Szl is caused by its frizzled domain, not the netrin domain.

(E) Sizzled inhibits Xlr proteolysis of Chordin via its frizzled domain.

(F) Sizzled is not digested by Xlr after 2 hr digestion with 2 nM Xlr enzyme.