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. Author manuscript; available in PMC: 2008 Jul 25.
Published in final edited form as: Chem Biol. 2005 Dec;12(12):1259–1268. doi: 10.1016/j.chembiol.2005.09.009

Figure 5. Inhibition of NFκB Transactivation Is Independent of the Presence of Calcium.

Figure 5

(A) HeLa cells were transfected with a κB-luciferase construct for all experimental conditions for 24 hr before the addition of 15 ng/ml TNF-α ± 100 nM triptolide. Cells were grown in the presence or absence of calcium-containing media for 16 hr before treatment and were then harvested for the assay after 6 hr. n = 4; data represented as mean ± SE. (B) At the same time as the κB-luciferase construct, cells were transfected with one of the following: GFP vector, NES-PV-GFP, NLSPV-GFP. They were the treated as described in (A). n = 4; data represented as mean ± SE.