Skip to main content
. Author manuscript; available in PMC: 2008 Jul 25.
Published in final edited form as: Chem Biol. 2005 Dec;12(12):1259–1268. doi: 10.1016/j.chembiol.2005.09.009

Figure 6. Triptolide Concentration Differentially Affects Viability/Growth or NFκB Inhibition.

Figure 6

(A) HeLa cells were plated at an initial concentration of 5 × 105 and were allowed to grow 6 triptolide (10−100 nM) for 48 hr. Viable (adherent) cells were photographed under 25× bright-field microscopy, and cell death was assessed by trypan dye exclusion. Results are representative of three separate experiments. (B) After a transient transfection with the κB-luciferase reporter construct, HeLa cells were incubated with 15 ng/ml TNF-α ± triptolide (10−100 nM) for a total of 6 hr before assessing reporter activity. n = 4; data represented as mean ± SE.