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. 2008 Jun 19;27(14):1919–1931. doi: 10.1038/emboj.2008.119

Figure 1.

Figure 1

Rictor is required for TM and HM phosphorylation of PKCα and Akt. (A) Deletion of Rictor gene decreases PKCα protein levels and phosphorylation. E10.5 embryos were lysed and used for immunoblot analysis. Immunoblotting was performed for PKCα and S6K phosphorylation states and mTOR complex components in lysates prepared from individual Rictor +/+, +/− and −/− embryos. (B) Phosphorylation of PKCα TM is eliminated in Rictor−/− embryos. Endogenous PKCα proteins were immunoprecipitated and a similar protein level was loaded. Phosphorylation of A-loop, TM and HM was determined by immunoblotting. (C) A-loop, TM and HM phosphorylation of transfected PKCα. HA–PKCα plasmid was transfected into Rictor+/+ or −/− MEF and HA–PKCα protein was immunoprecipitated and probed for phosphorylation. (D) Deletion of Rictor gene eliminates Akt TM and HM phosphorylation. E10.5 embryos were analysed for Akt phosphorylation.