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. 2008 Jun 26;27(14):2018–2029. doi: 10.1038/emboj.2008.123

Figure 1.

Figure 1

Interactions of Isl1, Lhx3 and Ldb1 in developing V2 interneurons and motor neurons. (A) Schematics of Isl1, Lhx3 and Ldb1 from mice showing the arrangement of key domains. In addition to the LIM and HD domains, Isl1 contains the Lhx3-binding domain (LBD; residues 262–291; identified herein) and the Isl-specific domain (ISD, identified on the basis of sequence conservation 296–310; Thor and Thomas, 1997). (B) Relative arrangement of Ldb1 and Lhx3 in developing V2 interneurons (left) and Ldb1, Lhx3 and Isl1 in postmitotic motor neurons (right). The interactions examined in this paper are indicated with dashed circles (1, 2, 3). (C) Far-UV CD spectra for Isl1LBD and Ldb1LID at concentrations of ∼ 20 μM. Raw baseline-corrected data are presented. (D) Y2H data comparing the interaction of Isl1 (residues 133–349) with Lhx3LIM1+2 in the absence or presence of Ldb1LID. The selection conditions used were -L-W-H, 1 mM 3-AT.