Table 1.
Crystallographic statistics for the Ras/NORE1A complex
| Data collection | Refinement | ||
|---|---|---|---|
| Space group | C2 | Resolution range (Å)a | 10–1.8 |
| Unit cell | Rwork (%)b | 19.3 | |
| a (Å) | 79.6 | Rfree (%)c | 23.0 |
| b (Å) | 88.0 | Number of atoms | |
| c (Å) | 56.5 | Protein | 2396 |
| β (deg) | 125.0 | GppNHp/Mg2+ | 32/1 |
| X-ray source | ESRF ID14-4 | Number of water molecules | 115 |
| Wavelength (Å) | 0.933 | Average B factor (Å2) | 25.8 |
| Resolution range (Å)a | 10–1.78 (1.85–1.78) | RMS deviations from ideal values | |
| Number of total reflections | 30 212 | Bond lengths (Å) | 0.015 |
| Number of unique reflections | 29 894 | Bond angles (deg) | 1.456 |
| Completeness (%)a | 97.2 (86.3) | Ramachandran plot | |
| I/σa | 13.6 (4.1) | Residues in most favoured regions (%) | 94.4 |
| Rmerge (%)a,d | 8.6 (34.8) | Residues in additional allowed regions (%) | 5.6 |
| aValues in parentheses correspond to the highest resolution shell. | |||
| bRwork=∑hkl∣Fo−Fc∣/∑hkl∣Fo∣, where Fo and Fc are the observed and calculated structure factors, respectively. | |||
| cRfree=∑hkl∣Fo−Fc∣/∑hkl∣Fo∣ was calculated with 5% of the data omitted from structure refinement. | |||
| dRmerge(I)=∑hkl∑i∣Ihkl,i−〈Ihkl〉∣/∑hkl∑i/Ihkl,i∣, where 〈Ihkl〉 is the average intensity of the multiple Ihkl,i observations for symmetry-related reflections. | |||