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Identification of the recombinant plasmid pIRES2-EGFP-HBs. (a) PCR result of HBs gene before transformation. M: Markers; 1: HBs gene; 2: negative control. (b) PCR result of HBs gene after transformation. M: Markers; 1 and 2: HBs gene; 3: negative control after transformation. (c) Result of enzyme digestion. M: Markers; 1 and 2: pRIS2-EGFP-HBs digested using EcoiR I and Sal I after transformation; 3: pRIS2-EGFP empty as native control.
