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. 2008 Jun 19;27(13):1863–1874. doi: 10.1038/emboj.2008.115

Figure 7.

Figure 7

RASSF1A disrupts MDM2–DAXX–HAUSP interactions and is required for MDM2 self-ubiquitination upon DNA damage. (A, B) U2OS cells were treated with MG132 for 6 h and then with adriamycin (1 μM) for the indicated times. MDM2–DAXX–HAUSP interactions (A) were analysed by immunoprecipitation (IP) with IgG (lane 1) and MDM2 (lane 2–8), followed by immunoblotting for ubiquitinated MDM2 (B). Molecular weights are in kDa. (C, D) U2OS cells transfected with GFP siRNA or RASSF1A siRNA (C), or Rassf1a+/+, Rassf1a−/− and Rassf1a−/− MEFs complemented with pcDNA-RASSF1A (D), were treated with adriamycin (1 μM) for 30 min. Mdm2-–Daxx–Hausp interactions were analysed by immunoprecipitation (IP) with the indicated antibodies (left) followed by immunoblotting for ubiquitinated Mdm2 (right). Molecular weights are in kDa.