Table 1.
CCT–3CBP interacting proteins in the absence of ATP, identified by LC-ESI-MS
| Gene name | Score | Mass (Da) | Polypeptide | Copies per cell | Synthesis rate |
|---|---|---|---|---|---|
| TCP1/CCT1 | 5511 | 60 899 | Chaperonin subunit | ND | 0.41 |
| CCT2 | 4409 | 57 510 | Chaperonin subunit | ND | 0.38 |
| CCT5 | 3010 | 61 152 | Chaperonin subunit | ND | 0.81 |
| CCT8 | 2976 | 61 965 | Chaperonin subunit | 2200 | 0.34 |
| CCT3 | 2929 | 59 404 | Chaperonin subunit | ND | 0.48 |
| CCT7 | 2924 | 60 211 | Chaperonin subunit | ND | ND |
| CCT4 | 2554 | 57 967 | Chaperonin subunit | 5530 | 0.34 |
| CCT6 | 2413 | 60 171 | Chaperonin subunit | ND | 0.42 |
| VID27 | 955 | 89 190 | WD40 (yeast/plants) | 3500 | 0.16 |
| TUB2 | 574 | 51 233 | β-Tubulin | ND | 0.40 |
| ACT1 | 201 | 41 906 | Actin | 215 000 | 2.30 |
| PLP1 | 180 | 26 834 | Phosducin-like protein 1 | 2250 | 0.15 |
| RPL23A | 165 | 14 578 | 60S ribosomal subunit | ND | 3.39 |
| CDC12 | 147 | 46 753 | Septin | 1170 | 0.23 |
| CDC3 | 108 | 60 188 | Septin | ND | 0.22 |
| CDC11 | 105 | 47 790 | Septin | 9280 | 0.16 |
| CDC10 | 70 | 37 059 | Septin | 14 100 | 0.26 |
| RPB7 | 58 | 24 330 | RNA pol II subunit 16 | 6420 | ND |
| RTT102 | 43 | 21 177 | SWI/SNF and RSC complex | 1550 | 0.16 |
| EFT2 | 39 | 93 660 | Diphthamide/His target | 160 782 | 2.37 |
| YCF1 | 38 | 172 196 | ABC transporter (cadmium) | 396 | 0.05 |
| PLP2 | 36 | 32 887 | Phosducin-like protein 2 | 7700 | 0.22 |
| EAF5 | 36 | 32 887 | NuA4 acetyltransferase | 937 | ND |
| FRS2 | 35 | 57 511 | Phenylalanyl-tRNA synthetase a | ND | 0.30 |
| YDR428C | 35 | 31 565 | Serine hydrolase | ND | ND |
| YNL155W | 34 | 31 953 | AN1-type zinc-finger | 4280 | ND |
| FAP1 | 33 | 113 668 | Transcription/binds FKBP12 | 589 | ND |
| ARP2 | 33 | 44 217 | Arp2/3 actin nucleation | 6650 | 0.35 |
| GRR1 | 33 | 133 848 | F-box SCF E3 ligase | ND | 0.03 |
| CSH1 | 33 | 44 639 | MIPC synthase | 195 | ND |
| PIF1 | 33 | 97 762 | DNA helicase mitochondrial | 259 | 0.06 |
| IRC10 | 32 | 68 217 | DNA repair | ND | ND |
| COX15 | 32 | 54 794 | Hydroxylation haeme O to haeme A | ND | ND |
| SPC34 | 32 | 34 171 | Dam1/DASH complex | ND | 0.10 |
| SHS1 | 31 | 62 706 | Septin | 5620 | 0.23 |
| RSR1 | 31 | 30 486 | GTPase (CDC24 interaction) | ND | ND |
| DPH1 | 30 | 48 679 | Diphthamide synthesis | 2562 | ND |
| NCBInr Saccharomyces cerevisiae database of 11 143 sequences was used for Mascot searches. CCT subunits (italics) and other proteins listed according to MudPIT score with a cutoff set at 30. Copy numbers are taken from the Swissprot database (http://expasy.org/sprot/), and estimated synthesis rate (in protein per second) are from Arava et al (2003). ND, not determined. | |||||