Table 3.

In vivo septin defects in cct mutant strains

Strain	Genotype	Temperature	Normal rings (%)		Abnormal rings (%)		Additional patches (%)		Total no. of cells analysed
			CDC3	CDC10	CDC3	CDC10	CDC3	CDC10	CDC3	CDC10
A. Septin localization in budded cells
MLY100	WT	15	98.2	100.0	1.8	0.0	0.0	1.0	110	100
		30	100.0	97.8	0.0	1.1	0.0	1.1	98	91
MLY110	plp1Δ	30	100.0	99.0	0.0	1.0	0.0	0.0	87	100
MLY111	pac10Δ plp1Δ	30	94.6	96.1	5.4	3.9	1.1	0.0	93	102
MLY118	pac10Δ	30	92.5	93.5	7.7	1.9	0.0	4.7	104	107
DUY558	CCT1	15	100.0	100.0	0.0	0.0	2.9	1.9	103	107
		25	94.5	99.1	5.5	0.9	1.8	0.9	109	113
		30	98.0	98.1	2.0	1.9	3.0	2.8	100	106
		37	98.2	98.2	1.8	0.0	1.8	1.8	112	111
DUY559	cct1-1	30	100.0	100.0	0.0	0.0	1.0	3.6	98	110
		15	55.6	62.0	44.4	38.0	18.8	12.0	117	108
DUY560	cct1-2	25	100.0	100.0	0.0	0.0	0.9	0.0	110	104
		37	96.2	100.0	3.8	0.0	1.9	0.0	106	101
DUY561	cct1-3	30	100.0	100.0	0.0	1.8	2.7	5.5	111	110
		15	96.0	76.6	4.0	23.4	1.0	29.0	101	107
CUY466	cct2-4	30	99.1	100.0	0.9	0.0	0.9	0.0	112	110
		15	94.1	98.1	5.9	1.9	4.0	3.8	101	106
CUY492	cct2-2	30	ND	ND	ND	ND	ND	ND	ND	ND
		15	ND	ND	ND	ND	ND	ND	ND	ND
CUY462	cct3-1	30	96.3	100.0	3.7	0.0	4.7	0.0	107	104
		15	92.3	94.6	7.7	5.4	3.3	2.2	91	93
DDY299	cct4-1	25	98.8	99.1	1.2	0.9	3.6	5.6	83	108
		37	84.6	82.4	15.4	17.6	14.1	11.0	78	91
B. Septin localization in unbudded cells
			Normal single patch (%)		Opposing extra patch (%)		Total no. of cells analysed
			CDC3	CDC10	CDC3	CDC10	CDC3	CDC10
MLY100	WT	30	100.0	100.0	0.0	0.0	77	57
DUY558	CCT1	25	91.1	93.8	8.9	6.2	79	81
		37	95.4	94.9	4.6	5.1	65	59
DDY299	cct4-1	25	100.0	100.0	0.0	0.0	54	95
		37	80.7	79.7	19.3	20.3	57	59
(A) Aberrant septin ring morphology or additional patches of GFP-septin signal were scored for the strains indicated at the noted temperatures. Scores differing significantly from wild type (P<0.01) are reported in bold. (B) The presence of additional septin patches in unbudded cct4-1 cells was scored and reported as in A. ND, not determined.