Figure 4. IL-4 and IL-13 enhance IFN-γ -induced IDO expression but reduce WRS expression in mouse microglia.
Microglia were stimulated with cytokines for 24 hrs and total RNA was analyzed for IDO (A, B) and WRS (C, D) expression by real time RT-PCR. Data are representative of six independent experiments with IL-4 and seven independent experiments with IL-13 done in triplicate. IDO expression in IFN-γ stimulated cells was significantly increased by treatment with IL-4 or IL-13, whereas IFN-γ stimulated WRS expression significantly reduced by IL-4 or IL-13.