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. Author manuscript; available in PMC: 2008 Aug 1.
Published in final edited form as: Virology. 2007 Apr 23;364(2):301–316. doi: 10.1016/j.virol.2007.03.037

Fig. 3. Effect of M1-si on dsRNA synthesis and infectious viral yields.

Fig. 3

(A) CV-1 cells were transfected with no siRNA or M1-si, and at 24 h p.t. were then infected with T3D reovirus at 10 PFU/cell. Cells were incubated for 24 or 48 h p.i., and samples were prepared for gel electrophoresis to detect the viral dsRNA genome segments. L, genome segments encoding λ proteins; M, genome segments encoding μ proteins; S, genome segments encoding σ proteins. (B, C) CV-1 cells were transfected with no siRNA, nonspecific control siRNA, or M1-si, and at 24 h p.t. were then infected with T1L (B) or T3D (C) reovirus at 10 PFU/cell. Cells were harvested at 0, 24, or 48 h p.i., and viral titers were determined by plaque assays. Log10 changes in viral titer relative to time 0 are indicated. Each bar represents the average obtained from three independent experiments, and error bars indicate the standard deviations.