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. 2008 May 23;59(10):2639–2647. doi: 10.1093/jxb/ern118

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© 2008 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

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Fig. 4. — Kdo-8-P synthesis in the T-DNA insertion AtkdsA1-S, AtkdsA2-S, and AtkdsA2-V null mutants. (a) Comparison of HPLC profiles obtained with a standard of Kdo derivatized with DMB, DMB-derivatized cytosolic monosaccharides extracted from wild-type WS A. thaliana leaves, wild-type Nicotiana tabacum leaves, and AtkdsA2-V mutant leaves. The arrow points out the specific peak of DMB-derivatized Kdo. (b) HPLC analysis of DMB-derivatized cytosolic monosaccharides extracted from AtkdsA1-S and AtkdsA2-S mutant leaves. The arrow points out the specific peak of DMB-derivatized Kdo, and the DMB-derivatized N-acetyl neuraminic acid (NeuAc), used as an internal standard for quantification of Kdo, is indicated.