Fig. 4.

BvCOL1 promoter/5′ UTR sequence and genetic map position. (A) BvCOL1 promoter/5′ UTR sequence. The 600 bp sequence upstream of the ATG start codon is shown in lower case letters. The positions of four single nucleotide polymorphisms in the K1 mapping population that were used for genetic mapping are underlined and in italics. The positions of primers used for SNP detection are indicated by arrows above the sequence. The asterisk indicates the 5′ end of the sequence corresponding to EST TC1427. Cis-regulatory DNA sequence motifs that are commonly found in circadian- and/or light-regulated genes are indicated as G-box (Martinez-Garcia et al., 2000; Michael and McClung, 2003), I-box (Terzaghi and Cashmore, 1995), SORLIP1 and 2 (Hudson and Quail, 2003) and CCAAT boxes (Wenkel et al., 2006). A 57 bp GA-rich tract that includes several GAGA sites and a GA octodinucleotide repeat motif (GA)₈, (Santi et al., 2003) just upstream of the translation start codon is underlined. (B) Using the four polymorphic sites indicated in (A), the position of BvCOL1 (arrow) was mapped on a SNP-based genetic map of expressed genes (Schneider et al., 2007). BvCOL1 is located on chromosome II between markers TG_E0536 and TG_E0022.