Fig. 2.

Zein–Nef accumulates to very low amounts in transgenic tobacco leaves. Proteins were extracted with reducing buffer from young leaves of plants expressing zein–Nef (lines 6 and 17, two independent transformants), zeolin, or wild-type tobacco (Co) and were analysed by SDS-PAGE followed by protein blot using anti-γ-zein antiserum. Extract containing 50 μg (wild-type and zein–Nef plants) or 10 μg (zeolin plant) of total protein was loaded in each lane. The positions of zein–Nef (arrow) and zeolin (asterisk) are marked on the right. Numbers on the left indicate the positions of molecular mass markers, in kilodaltons.