Fig. 7.

Phaseolin fragments accumulate in plants that synthesize zeolin–Nef. Proteins were extracted with reducing buffer from young leaves of independent lines of tobacco transformed with the vector encoding zeolin–Nef (the different trangenic lines are identified by single letters) or from wild-type tobacco (wt). Analysis was by SDS–PAGE followed by protein blot using anti-phaseolin antiserum. Equal amounts of protein extract (40 μg) were loaded in each lane. The two lanes between N and wt contain extracts from plants that were positive by antibiotic selection but negative for recombinant protein expression. The positions of intact zeolin (arrowhead) and phaseolin fragment (vertical line) are marked on the right. Numbers on the left indicate the positions of molecular mass markers, in kilodaltons.