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Zeolin–Nef forms protein bodies. Thin sections prepared from young leaves of transgenic tobacco expressing zeolin–Nef were incubated with anti-phaseolin antiserum (A, B, D), anti-Flag antibodies (C, E), or preimmune serum (F), followed by secondary goat anti-rabbit 15 nm gold complex. V, Vacuole. Bars = 500 nm (A, B, C, E, F) or 1000 nm (D).
