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. 1998 Nov 10;95(23):13612–13617. doi: 10.1073/pnas.95.23.13612

Figure 1.

Figure 1

Generation of FSH-R gene-deficient mice. (A) Strategy for generating the FSH-R null mutation. The EcoRV fragment containing exon 1 of the FSH-R gene was replaced by a PGK-Neo cassette. (B) DNA extracted from tail biopsies of wild-type (+/+), heterozygous (+/−), and homozygous (−/−) mice were digested with EcoRI; hybridization with probe 1 yielded 11-kb (wild type) and 9.5-kb (mutant) bands. (C) Northern analysis of poly(A)+ RNA extracted from mouse testis. (D) Reverse transcription–PCR from testis and ovary total RNA; primers used were selected within the sequence of exon 2 (sense primer: 5′-GTGCTCACCAAGCTTCGA-3′) and exon 7 (antisense primer: 5′-GAATCCCATTCTTATTCAGC-3′); all isoforms of the FSH-R are apparent. (E) FSH binding to crude membrane preparation obtained from testes of wild-type, heterozygous, and homozygous mice.