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Kinetics of phosphatidylserine (PS) flip (A), nuclear morphology (B), and DNA fragmentation (C) in apoptotic wild-type and caspase-3−/− thymocytes. Thymocytes from either wild-type or caspase-3−/− mice were cultured in RPMI medium (10% fetal bovine serum) at 37°C in the presence of coated anti-Fas antibody Jo2. At each indicated time point, cells were harvested and subjected to morphological analysis, PS flipping, and DNA fragmentation assay as described in Materials and Methods.
