In situ hybridization analysis of PTCH, PTCH2, and Fused. Emulsion autoradiographs after in situ hybridization of sagittal sections through embryonic day 18 (E18) mouse for PTCH (a) or PTCH2 (b) probes, transverse sections through E18 wild-type or SMO-M2 transgenic mouse skin for PTCH (c and e) and PTCH2 (d and f), and sections of adult mouse testis for PTCH (g), PTCH2 (h and k), Fused (i and l), and Dhh (j). [Bar = 2.1 mm (a and b); 0.065 mm (c– f, j); 0.12 mm (h and i); 1.0 mm (k and l).] bn, bone; gt, gut; sk, skin; th, teeth; vib, vibrissae; ep, epidermis; de, dermis; fol, hair follicle.