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. 1998 Nov 10;95(23):13641–13645. doi: 10.1073/pnas.95.23.13641

Figure 1.

Figure 1

(A) Copper dependence of iron transport in wild-type yeast and in Δgef1 cells. Wild-type (DY1457) and Δgef1 cells were grown in YPD overnight or in medium made iron limited by the addition of 80 μM bathophenanthrolinedisulfonate (BPS) for 4 h. (B) Fet3p-dependent PPD oxidase activity from wild-type and gef1 cells. Membranes from wild-type and gef1 cells were isolated either in the presence of the Cu chelator bathocuproinedisulfonic acid (BCS) or in the presence of additional Cu+. PPD oxidase activity of the isolated membranes was determined as described in Materials and Methods. The gef1–1 allele was derived from parental strain JY102, and the gef1–2 allele was derived from parental strain DY1457. (Upper) PPD oxidase activity. (Lower) Western blot, to show equal loading of Fet3p.