Figure 5.

Farnesylation in differentiated, K-ras, and H-ras transformed FRTL-5 cells. [³H]-farnesyl-PP labeling in whole-cell and cell-free experiments carried out with FRTL-5, KiMol, Ats (39 and 33°C), and H-ras cells, as described in Experimental Procedures. (A) Electrophoretic profile of farnesylated proteins labeled in whole-cells labeled with [³H]-farnesyl-PP by using low density lipoprotein as a carrier, and HPLC analysis of prenyl groups attached to proteins. These data are representative of three independent experiments. Molecular weight standards (45 and 21) are shown on the right. (B) p21^ras farnesylated in cell-free experiments carried out with normal, KiMol, and H-ras FRTL-5 cell homogenates. The autoradiograms were exposed for 20 days. The experiments were repeated with similar results.