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GFP-RLC-myosin rod is expressed at levels similar to those of wild-type myosin II in Dictyostelium cells. Whole cell lysates of cells expressing wild-type myosin II (lane 1), GFP-RLC-myosin rod (lane 2), or no myosin II (lane 3) were analyzed on an SDS/7.5% polyacrylamide gel. (Left) Coomassie brilliant blue staining. (Right) An immunoblot of the whole cell lysates using an anti-myosin II antibody demonstrates that the appropriate proteins are expressed in comparable amounts.
