Figure 1.

Carboxyl-terminal fragment of FAK (FRNK) inhibits invasin-mediated uptake. (A) The amount of bacterial internalization was determined for CEF cells that were transfected with either RCAS vector alone or RCAS overexpressing FAK or FRNK (Materials and Methods). %UPTAKE refers to the amount of internalization observed relative to that of the vector-transfected control. A representative experiment is shown with error bars indicating the SE for samples prepared in triplicate. For vector-transfected control, 1.3 ± 0.3% of the initial bacterial inoculum was internalized during the course of the experiment. (B) FRNK overexpression does not inhibit invasin-mediated binding to CEFs. The amount of bacterial binding was determined (Materials and Methods) for vector-transfected cells (filled bars) and FRNK overexpressing CEF cells (empty bars) of Giemsa-stained preparations.