Figure 3.

Tyr-397 substitution of FAK is unable to overcome FRNK inhibition of invasin-mediated uptake. (A) Bacterial internalization was determined for vector-transfected cells or cells overexpressing a mixture of FAK derivatives (Materials and Methods). FAK: wild-type FAK transfectants; FRNK: transfectants expressing FRNK interfering fragment; D562A or K464R: FAK derivatives lacking kinase activity; Y397F: FAK substitution lacking autophosphorylation site. %UPTAKE refers to the amount of internalization observed relative to that of the vector-transfected control. (B) Cell lysates of vector-transfected cells or cells overexpressing FAK derivatives were examined by immunoblotting with anti-FAK serum. Samples were identical to those in A.